Basic Information:

Symbol	Stim1
Synonyms	Sim
Species	Mouse
Entrez ID	20866
Uniprot ID	P70302
Membrane Contact Site	ER-PM; PM-ER
Cell line/Tissue	MEFs
Experimental Method	Low throughput experimental methods
Protein Sequence

Complex Information:

Complex ID	Subunit of complex	Subcellular location	Species	More
CMCS00006	ZFYVE27; FYCO1; KIF5B; RAB7A; PI(3)P	ER-Endosome; Endosome-ER	Human	more
CMCS00063	Orai1; Stim1; Unc93b1	ER-PM; PM-ER	Mouse	more
CMCS00153	Sec22b; Stim1	ER-PM; PM-ER	Mouse	more

Expression Overview of Stim1:

Homology Information of Stim1:

Uniprot ID	P70302
EggNOG	KOG4403
HOGENOM	CLU_010588_0_0_1
OrthoDB	54068at2759
TreeFam	TF313487
GeneTree	ENSGT00390000000214

References:

DOI	https://doi.org/10.1101/2022.04.12.487993
Description	Total internal reflection microscopy (TIRF) analysis allowed simultaneous imaging of Sec22b and STIM1 recruitment to ER-PM MCS, visible as puncta at the PM plane, during the activation of store-operated Ca2+ entry (SOCE) .
Description of experimental evidence	The protein was validated by immunofluorescence , EM, CLEM, calcium imaging , TIRF imaging , PLF assay, immunoblotting, immunoprecipitation and flow cytometry in MEFs.
More related results

Pubmed ID	35065962
DOI	10.1016/j.jbc.2022.101607
Description	At these ER–PM junctions, STIM1 can trap and gate Orai1 Ca2+ channels, eliciting an influx of Ca2+ from the extracellular milieu into the cytoplasm.; UNC93B1 deficiency might impair the traffic or activity of an adapter protein to reduce STIM1–Orai1 interactions but not STIM1 recruitment.
Description of experimental evidence	The protein was validated by immunoprecipitation, western blot analysis, PCR ,qPCR, Duolink and TIRF microscopy in HEK293T cells, which maintains the ER calcium sensor STIM1 in a dimeric state primed for translocation to the ER cortex.More related results
More related results