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Basic Information:

Symbol	ZFYVE27
Synonyms	SPG33; Protrudin
Protein Name	Protrudin (Spastic paraplegia 33 protein) (Zinc finger FYVE domain-containing protein 27)
Species	Human
Entrez ID	118813
Uniprot ID	Q5T4F4
Membrane Contact Site	ER-Endosome; Endosome-ER
Location (from literature)	ER
Cell line/Tissue	HeLa cells; hTERT-RPE1 cells; HL60 cells; HEK293 cells
Experimental Method	Low throughput experimental methods
Protein Sequence
More related results

Complex Information:

Complex ID	Subunit of complex	Subcellular location	Species	More
CMCS00006	ZFYVE27; FYCO1; KIF5B; RAB7A; PI(3)P	ER-Endosome; Endosome-ER	Human	more
CMCS00183	PDZD8; RAB7A; ZFYVE27	ER-Endosome; Endosome-ER	Human	more

Expression Overview of ZFYVE27:

Homology Information of ZFYVE27:

Uniprot ID	Q5T4F4
EggNOG	ENOG502QVKC
HOGENOM	CLU_060341_0_0_1
OrthoDB	38671at2759
TreeFam	TF331044
GeneTree	ENSGT00390000013298

References:

Pubmed ID	25855459
DOI	10.1038/nature14359
Description	Here we show in human and rat cell lines that protrudin, an ER protein that promotes protrusion and neurite outgrowth, forms contact sites with late endosomes (LEs) via coincident detection of the small GTPase RAB7 and phosphatidylinositol 3-phosphate (PtdIns(3)P). These contact sites mediate transfer of the microtubule motor kinesin 1 from protrudin to the motor adaptor FYCO1 on LEs.
Description of experimental evidence	The protein was validated by microscopy, western blotting, GFP-trap assay, in HeLa cells,hTERT-RPE1 cells, hTERT-RPE1 cells and HL60 cells, and these results correlate strongly with the cooperative effects of protrudin and FYCO1 in protrusion and neurite outgrowth.
More related results

Pubmed ID	32686675
DOI	10.1038/s41467-020-17451-7
Description	Our results identify the ER membrane proteins, PDZD8 and Protrudin, together with the late endosomal protein Rab7, as components of an ER-late endosome MCS in mammalian cells.
Description of experimental evidence	The protein was validated by immunofluorescence, spinning-disc confocal microscopy, immunopurifications, immunoprecipitation analysis and mass spectrometry analysis in HEK293T cells and U2-OS cells, which functions to regulate late endosomal motility and distribution, which is intimately linked to endosomal maturation.
More related results