You can enter information about the chemical modification sequence of interest without the need for the original sequence. If there is raw sequences, the results will be more accurate.

Click the example button could provide format of sequence and chemical modification.
Batch analysis as well as mixed inputs with different modifications are supported !

Sequence Format Guide:

  • Introduction: our system will automatically identify and split the modification type of each nucleotide on each modification unit on the sequence and give detailed annotation information, so as to help you quickly and accurately locate the chemical modification you are interested in.
  • RAW siRNA sequence: Please type in given area (fastq format) as followed. Note: sequence order of sense strand and anti-sense strand are both 5'-3'.For example:
    Sense strand: AUGCUGAAACAUGCAGUUGUA
    Antisense strand: UACAACTGCAUGUUUCAGCAUCU
  • Chemical modifications: Use uppercase for standard nucleotides (A/U/C/G).Methylation modifications should be lowercase (a/u/g/c). Mixed inputs for different grooming types are supported (e.g. Af=2'-fluoro-adenosine, s=phosphorothioate. (Tgn)=Glycol Nucleic Acid thymidine. a=2'-O-Methyladenosine.) Separate domains with hyphens. Avoid typos in lowercase/uppercase and unmatched brackets.
    For example:
    Sense strand: asusgcugAfaAfCfAfugcaguugsusa
    Antisense strand: VPuAfcadAc(Tgn)gcauguUfuCfagcauscsu
  • Rare specialized modification types: Our system supports more than 40 different modifications, but if there is a very rare type of special modification, please fill it out separately in the text box in the bottom right corner. (e.g. (C11-PEG3-NAG3), (J2-CONC16A).)

siRNA sequence:

Example

Chemical Modification and position:

Example

Rare specialized modification types:(Optional)

Example

Contact zhy1001@alu.uestc.edu.cn or yangzhang@cdutcm.edu.cn
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