Detail Information of Protein
Basic Information:
| Symbol | Itpr1 |
| Synonyms | Insp3r; Pcd6; Pcp1; IP3R |
| Protein Name | Inositol 1,4,5-trisphosphate receptor type 1 (IP3 receptor isoform 1) (IP3R 1) (InsP3R1) (Inositol 1,4,5-trisphosphate-binding protein P400) (Protein PCD-6) (Purkinje cell protein 1) (Type 1 inositol 1,4,5-trisphosphate receptor) (Type 1 InsP3 receptor) |
| Species | Mouse |
| Entrez ID | 16438 |
| Uniprot ID | P11881 |
| Membrane Contact Site |
ER-MT; MT-ER
|
| Location (from literature) | ER |
| Cell line/Tissue | Mouse livers; MEFs; Mouse testes; Mouse brains and retinas; RGCs; HRECs; C2C12 myoblasts |
| Experimental Method | Low throughput experimental methods |
| Protein Sequence | |
| More related results |
Complex Information:
| Complex ID | Subunit of complex | Subcellular location | Species | More |
| CMCS00161 | Tgm2; Vdac1; Itpr1; Hspa9 | ER-MT; MT-ER | Mouse | more | CMCS00175 | Vdac1; Itpr1; Hspa9 | ER-MT; MT-ER | Mouse | more | CMCS00266 | Itpr3; Hspa9; Vdac1; Pdk4 | ER-MT; MT-ER | Mouse | more |
Expression Overview of Itpr1:
Homology Information of Itpr1:
| Uniprot ID | P11881 |
| EggNOG | KOG3533 |
| HOGENOM | CLU_000206_1_0_1 |
| OrthoDB | 5480299at2759 |
| TreeFam | TF312815 |
| GeneTree | ENSGT00940000155071 |
References:
| Pubmed ID | 30590033 |
| DOI | 10.1016/j.celrep.2018.11.094 |
| Description | GRP75 localizes in Mitochondrion-associated membranes (MAMs) and acts as a bridging molecule between the two organelles by assembling the IP3R-GRP75-VDAC complex, which is involved in the transport of Ca2+from the endoplasmic reticulum (ER) to Mitochondrion. |
| Description of experimental evidence | The protein was validated by western blot analysis, Co-IP and PLA in mouse livers and MEFs. |
| More related results |
| Pubmed ID | 29785746 |
| DOI | 10.1002/pmic.201700478 |
| Description | By Andromeda search engine against the decoy UniProt-mouse database and Swiss-Prot human database, which yielded 2,808 proteins from mouse testes (mT),2,478 proteins from mouse brain (mB) and 2,155 proteins from human testes (hT). |
| Description of experimental evidence | The protein was validated by TEM and LC -MS/MS analyses in mouse testes and mouse brains. |
| More related results |
| Pubmed ID | 28522876 |
| DOI | 10.1038/s41598-017-02213-1 |
| Description | Using this method, 1313 non-redundant proteins were identified in the MAM. |
| Description of experimental evidence | The protein was validated by proteomic analysis, long gradient nano-reverse-phase liquid chromatography, mass spectrometry and bioinformatics analysis in RGCs, HRECs, mouse brains and retinas. |
| More related results |
| Pubmed ID | 24947355 |
| DOI | 10.2337/db13-1751 |
| Description | Mitochondria-associated endoplasmic reticulum (ER) membranes (MAMs) are functional domains between both organelles involved in Ca2+ exchange, through the voltage-dependent anion channel (VDAC)-1/glucose-regulated protein 75 (Grp75)/inositol 1,4,5-triphosphate receptor (IP3R)-1 complex, and regulating energy metabolism. |
| Description of experimental evidence | The protein was validated by immunofluorescence, duolink II in situ PLA and western blot in mouse livers, which regulates energy metabolism. |
| More related results |
| Pubmed ID | 30523025 |
| DOI | 10.2337/db18-0363 |
| Description | Here, we demonstrate that PDK4 interacts with and stabilizes the IP3R1-GRP75-VDAC1 complex at the MAM interface. |
| Description of experimental evidence | The protein was validated by in situ proximity ligation assay, immunofluorescence analysis, coimmunoprecipitation, transmission electron microscopy and immunoblotting in C2C12 myoblasts, which augments ER–Mitochondria contact to dampen skeletal muscle insulin signaling during obesity. |
| More related results |