Basic Information:

Symbol	STARD3
Synonyms	CAB1; MLN64
Species	Human
Entrez ID	10948
Uniprot ID	Q14849
Membrane Contact Site	ER-Endosome; Endosome-ER
Location (from literature)	Endosome
Cell line/Tissue	HeLa cells; HEK293T cells
Experimental Method	Low throughput experimental methods
Protein Sequence

Complex Information:

Complex ID	Subunit of complex	Subcellular location	Species	More
CMCS00014	MOSPD2; STARD3	ER-Endosome; Endosome-ER	Human	more
CMCS00023	VAPA; VAPB; STARD3	ER-Endosome; Endosome-ER	Human	more

Expression Overview of STARD3:

Homology Information of STARD3:

Uniprot ID	Q14849
EggNOG	KOG3845
HOGENOM	CLU_033480_0_0_1
OrthoDB	3740290at2759
TreeFam	TF313869
GeneTree	ENSGT00940000159051

References:

Pubmed ID	24105263
DOI	10.1242/jcs.139295
Description	STARD3 or STARD3NL and VAP form a novel molecular tether between late endosomes and the ER.
Description of experimental evidence	The protein was validated by immunoprecipitation, electron microscopy, immunofluorescence, in situ proximity ligation assay, mSDS-PAGE and western blot analysis in HeLa cells.
More related results

Pubmed ID	28377464
DOI	10.15252/embj.201695917
Description	Corroborating this, in vitro reconstitution assays indicated that STARD3 and its ER-anchored partner, Vesicle-associated membrane protein-associated protein (VAP), assemble into a machine that allows a highly efficient transport of cholesterol within membrane contacts.
Description of experimental evidence	The protein was validated by immunofluorescence, colocalization analysis, filipin staining, GFP-D4, filipin co-staining, flotation experiments, electron microscopy and stereology in Hela cells, and the complex allows a highly efficient transport of cholesterol within membrane contacts.
More related results

Pubmed ID	29858488
DOI	10.15252/embr.201745453
Description	Consequently, MOSPD2 and these organelle‐bound proteins mediate the formation of contact sites between the ER and endosomes, mitochondria, or Golgi.; ll these proteins, by binding VAP proteins, are known to build contact sites between the ER and endosomes (STARD3, STARD3NL, ORP1L), mitochondria (PTPIP51), and Golgi (STARD11).
Description of experimental evidence	The protein was validated by immunofluorescence, colocalization analysis, SDS–PAGE, western blot, coomassie blue staining, pull‐down assays, GFP‐Trap, mass spectrometry analysis, electron microscopy and immunoprecipitation in HeLa cells and HEK293T cells.
More related results