Basic Information:

Symbol	PDZD8
Synonyms	PDZK8
Species	Human
Entrez ID	118987
Uniprot ID	Q8NEN9
Membrane Contact Site	ER-Endosome; Endosome-ER
Location (from literature)	ER
Cell line/Tissue	HEK293 cells; HeLa cells; HEK293T cells; COS-7 cells; U2-OS cells
Experimental Method	Low throughput experimental methods
Protein Sequence

Complex Information:

Complex ID	Subunit of complex	Subcellular location	Species	More
CMCS00018	PDZD8; RAB7A	ER-Endosome; Endosome-ER	Human	more
CMCS00183	PDZD8; RAB7A; ZFYVE27	ER-Endosome; Endosome-ER	Human	more

Expression Overview of PDZD8:

Homology Information of PDZD8:

Uniprot ID	Q8NEN9
EggNOG	KOG3532
HOGENOM	CLU_008594_0_0_1
OrthoDB	3681901at2759
TreeFam	TF324166
GeneTree	ENSGT00390000017746

References:

Pubmed ID	31636202
DOI	10.1073/pnas.1913509116
Description	PDZD8 mediates a Rab7-dependent interaction of the ER with late endosomes and lysosomes.
Description of experimental evidence	The protein was validated by microscopy, immunofluorescence and pull-down assay in HEK293 cells and HeLa cells.
More related results

Pubmed ID	33912962
DOI	10.1242/jcs.255026
Description	PDZD8-mediated lipid transfer at contacts between the ER and late endosomes/lysosomes is required for neurite outgrowth.
Description of experimental evidence	The protein was validated by confocal microscopy, live cell super-resolution Lattice-SIM microscopy, immunofluorescence staining, quantitative RT-PCR, GFP-trap assay, mass spectrometry, pulldown assays and pull-down assay in HeLa cells, HEK293T cells, COS-7 cells and U2-OS cells, which is required for LE/lys positioning and neurite outgrowth, which is dependent on the lipid transfer activity of the SMP domain.
More related results

Pubmed ID	32686675
Description	Our results identify the ER membrane proteins, PDZD8 and Protrudin, together with the late endosomal protein Rab7, as components of an ER-late endosome MCS in mammalian cells.
Description of experimental evidence	The protein was validated by immunofluorescence, spinning-disc confocal microscopy, immunopurifications, immunoprecipitation analysis and mass spectrometry analysis in HEK293T cells and U2-OS cells, which functions to regulate late endosomal motility and distribution, which is intimately linked to endosomal maturation.
More related results