MCS.ID	synonyms	gene.ID	gene.symbol	uniprot	species	MCS	location.literature.	cell.line.tissue	Experimental.Method	source.number	PMID-1	Description-1	Evidences-1	PMID-2	Description-2	Evidences-2	PMID-3	Description-3	Evidences-3	PMID-4	Description-4	Evidences-4	PMID-5	Description-5	Evidences-5	PMID-6	Description-6	Evidences-6	PMID-7	Description-7	Evidences-7	PMID-8	Description-8	Evidences-8	PMID-9	Description-9	Evidences-9	PMID-10	Description-10	Evidences-10	PMID-11	Description-11	Evidences-11	PMID-12	Description-12	Evidences-12
LMCS00107	Slc11a2; Dct1; Dmt1; Nramp2	25715	Slc11a2	O54902	Rat	ER-Endosome; Endosome-ER	MT	Rat renal cortex	Low throughput experimental methods	1	29317744	Nevertheless, it is attractive to speculate that endosomal DMT1 interacting with OMM DMT1 contributes not only to the docking but also provides a source of protons to drive the process.	The protein was validated by metal transport assays, radioisotope tracers, PGSK fluorescence, immunoblotting and protein determination in rat renal cortex, which have a role in mitochondrial Fe2+ and Mn2+ acquisition.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00108	Mttp; Mtp	310900	Mttp	D4A1W8	Rat	ER-MT; MT-ER	ER	Rat livers	Low throughput experimental methods	1	7961664	In addition, the microsomal triacylglycerol transfer protein, which is required for the assembly secretion of apolipoprotein B-containing lipoproteins, was present in the MAM.	The protein was validated by immunocytochemistry, electron microscopy and conventional electron microscopy in rat livers.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00111	Pigbos1	100909598	Pigbos1	C0HLN0	Rat	ER-MT; MT-ER	MT	Rat C6 cells; Rat tissues	Low throughput experimental methods	1	31653868	This result demonstrated that the PIGBOS-CLCC1 interaction can be regulated by modulation of ER-mitochondria contacts, and further bolstered the model of CLCC1 and PIGBOS interacting at ER-mitochondria contact sites.	The protein was validated by confocal imaging, APEX labeling in live cells, immunoprecipitations, flow cytometry analysis, western blot analysisand EM in rat C6 cells and rat tissues, and the loss of PIGBOS leads to heightened UPR and increased cell death.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00112	Clcc1; Mclc	170927	Clcc1	Q9WU61	Rat	ER-MT; MT-ER	ER	Rat C6 cells; Rat tissues	Low throughput experimental methods	1	31653868	This result demonstrated that the PIGBOS-CLCC1 interaction can be regulated by modulation of ER-mitochondria contacts, and further bolstered the model of CLCC1 and PIGBOS interacting at ER-mitochondria contact sites.	The protein was validated by confocal imaging, APEX labeling in live cells, immunoprecipitations, flow cytometry analysis and EM in HEK293 cells, HEK293T cells, U2-OS cells, COS-7 cells and HeLa cells, and the loss of PIGBOS leads to heightened UPR and increased cell death.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00113	Amfr	267	Amfr	A0A0G2K437	Rat	ER-MT; MT-ER	NA	Rat livers; MDCK II cells	Low throughput experimental methods	1	10995452	By EM, smooth ER AMF-R tubules exhibit direct interactions with Mitochondrion.	The protein was validated by confocal and electron microscopy in rat livers and MDCK II cells.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00121	Hspa9; Grp75; Hspa9a	291671	Hspa9	P48721	Rat	ER-MT; MT-ER	MT	Rat livers	Low throughput experimental methods	1	17178908	VDAC1, grp75, and IP3Rs are present in a macromolecular complex at the ER-mitochondria interface.	The protein was validated by subcellular fractionation, proteomic analysis, targeted aequorin probes and Imaging techniques in rat livers, which directly enhanced Ca2+ accumulation in mitochondria.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00122	Vdac1	83529	Vdac1	Q9Z2L0	Rat	ER-MT; MT-ER	MT	Rat livers	Low throughput experimental methods	1	17178908	VDAC1, grp75, and IP3Rs are present in a macromolecular complex at the ER-mitochondria interface.	The protein was validated by subcellular fractionation, proteomic analysis, targeted aequorin probes and Imaging techniques in rat livers, which directly enhanced Ca2+ accumulation in mitochondria.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00130	Snca	29219	Snca	P37377	Rat	ER-MT; MT-ER	NA	Rat cortical neurons; Rat brains	Low throughput experimental methods	1	28337542	alpha-Synuclein is a MAM protein and binds to VAPB but not PTPIP51 in immunoprecipitation assays.	The protein was validated by SDS-PAGE, immunoblotting, immunoprecipitation and GST pull-down assays, biochemical fractionation and microscopy in rat cortical neuronsand rat brains, which disrupts Ca2+ homeostasis and mitochondrial ATP production.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00159	Rmdn3; Fam82a2; Fam82c; Ptpip51	311328	Rmdn3	Q66H15	Rat	ER-MT; MT-ER	MT	Hippocampal neurons; Rat cortical neurons; Rat brains	Low throughput experimental methods	3	30841933	The VAPB-PTPIP51 endoplasmic reticulum-mitochondria tethering proteins are present in neuronal synapses and regulate synaptic activity	The protein was validated by protein fractionation, SDS–PAGE, immunoblotting,immunofluorescence staining, proximity ligation assays and microscopy in hippocampal neurons, and the results reveal a new role for the VAPB-PTPIP51 tethers in neurons and suggest that damage to ER-Mitochondrion signaling contributes to synaptic dysfunction in Parkinson's disease and FTD or ALS..	28337542	However, many neuronal functions damaged in Parkinson’s disease are regulated by signalling between the endoplasmic reticulum (ER) and mitochondria. This signalling involves close physical associations between the two organelles that are mediated by binding of the integral ER protein vesicle-associated membrane protein-associated protein B (VAPB) to the outer mitochondrial membrane protein, protein tyrosine phosphatase-interacting protein 51 (PTPIP51). VAPB and PTPIP51 thus act as a scaffold to tether the two organelles.	The protein was validated by SDS-PAGE, immunoblotting, immunoprecipitation and GST pull-down assays, biochemical fractionation and microscopy in rat cortical neuronsand, rat brains, and alpha-Synuclein binds to the ER–mitochondria tethering protein VAPB to disrupt Ca2+ homeostasis and mitochondrial ATP production.	35090998	Lanthanum decreased VAPB-PTPP51, BAP31-FIS1, and MFN2-MFN1 expression of mitochondria-associated membranes and induced abnormal autophagy in rat hippocampus.	The protein was validated by morris water maze, transmission electron microscopy, nissl staining, immunofluorescence test , real-time quantitative PCR and western blot analysis in rat hippocampal cells and tissues.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00160	Vapb	60431	Vapb	Q9Z269	Rat	ER-MT; MT-ER	ER	Hippocampal neurons; Rat cortical neurons; Rat brains	Low throughput experimental methods	3	30841933	Here, we demonstrate that the VAPB-PTPIP51 tethers regulate synaptic activity.VAPB and PTPIP51 localise and form contacts at synapses, and stimulating neuronal activity increases ER-Mitochondrion contacts and the VAPB-PTPIP51 interaction.Moreover, siRNA loss of VAPB or PTPIP51 perturbs synaptic function and dendritic spine morphology.Our results reveal a new role for the VAPB-PTPIP51 tethers in neurons and suggest that damage to ER-Mitochondrion signaling contributes to synaptic dysfunction in Parkinson's disease and FTD or ALS.	The protein was validated by protein fractionation, SDS–PAGE, immunoblotting,immunofluorescence staining, proximity ligation assays and microscopy in hippocampal neurons, and the results reveal a new role for the VAPB-PTPIP51 tethers in neurons and suggest that damage to ER-Mitochondrion signaling contributes to synaptic dysfunction in Parkinson's disease and FTD or ALS..	28337542	alpha-Synuclein is a MAM protein and binds to VAPB but not PTPIP51 in immunoprecipitation assays.	The protein was validated by SDS-PAGE, immunoblotting, immunoprecipitation and GST pull-down assays, biochemical fractionation and microscopy in rat cortical neuronsand, rat brains, and alpha-Synuclein binds to the ER–mitochondria tethering protein VAPB to disrupt Ca2+ homeostasis and mitochondrial ATP production.	35090998	Lanthanum decreased VAPB-PTPP51, BAP31-FIS1, and MFN2-MFN1 expression of mitochondria-associated membranes and induced abnormal autophagy in rat hippocampus.	The protein was validated by morris water maze, transmission electron microscopy, nissl staining, immunofluorescence test , real-time quantitative PCR and western blot analysis in rat hippocampal cells and tissues.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00161	Panx2; Px2	362979	Panx2	P60571	Rat	ER-MT; MT-ER	ER	C6 glioma cells	Low throughput experimental methods	1	30862038	Pannexin 2 localizes at ER-Mitochondria contact sites.	The protein was validated by live-cell imaging, panx2 trajectory analysis, immunofluorescence, co-Localization analysis, pre-pmbedding immunoelectron microscopy and western blotting in C6 glioma cells, which sensitizes cells to apoptotic stimuli.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00169	Fis1; Ttc11	288584	Fis1	P84817	Rat	ER-MT; MT-ER	MT	Rat hippocampal cells and tissues	Low throughput experimental methods	1	35090998	Lanthanum decreased VAPB-PTPP51, BAP31-FIS1, and MFN2-MFN1 expression of mitochondria-associated membranes and induced abnormal autophagy in rat hippocampus.	The protein was validated by morris water maze, transmission electron microscopy, nissl staining, immunofluorescence test , real-time quantitative PCR and western blot analysis in rat hippocampal cells and tissues.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00170	Bcap31	293852	Bcap31	Q6AY58	Rat	ER-MT; MT-ER	ER	Rat hippocampal cells and tissues	Low throughput experimental methods	1	35090998	Lanthanum decreased VAPB-PTPP51, BAP31-FIS1, and MFN2-MFN1 expression of mitochondria-associated membranes and induced abnormal autophagy in rat hippocampus.	The protein was validated by morris water maze, transmission electron microscopy, nissl staining, immunofluorescence test , real-time quantitative PCR and western blot analysis in rat hippocampal cells and tissues.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00171	Acsl4; Acs4; Facl4	113976	Acsl4	O35547	Rat	ER-MT; MT-ER	ER; MT	Rat livers	Low throughput experimental methods	2	11319232	We have previously shown that in rat liver, ACS1 is located in ER, mitochondrial-associated membrane (MAM), and cytosol, ACS4 is located in MAM.	The protein was validated by immunoblotting and immunoblotting in rat livers, which may be linked to triacylglycerol synthesis.	12147264	Rat liver acyl-CoA synthetase 4 is a peripheral-membrane protein located in two distinct subcellular organelles, peroxisomes, and Mitochondrionl-associated membrane.	The protein was validated by northern analyses, immunoblotting and enzyme assays in rat livers, which is probably targeted and linked to MAM and peroxisomes by interactions with other proteins.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00172	Acsl1; Acs1; Acsl2; Facl2	25288	Acsl1	P18163	Rat	ER-MT; MT-ER	ER	Rat livers	Low throughput experimental methods	2	11319232	We have previously shown that in rat liver, ACS1 is located in ER, mitochondrial-associated membrane (MAM), and cytosol, ACS4 is located in MAM.	The protein was validated by immunoblotting and immunoblotting in rat livers, which may be linked to triacylglycerol synthesis.	12147264	ACS1 had been localized to three different subcellular regions in rat liver, endoplasmic reticulum, Mitochondrion, and peroxisomes.	The protein was validated by northern analyses, immunoblotting and enzyme assays in rat livers.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00175	Itpr3	25679	Itpr3	Q63269	Rat	ER-MT; MT-ER	ER	Rat livers	Low throughput experimental methods	1	17178908	VDAC1, grp75, and IP3Rs are present in a macromolecular complex at the ER-mitochondria interface. 	The protein was validated by subcellular fractionation, proteomic analysis, targeted aequorin probes and Imaging techniques in rat livers, which directly enhanced Ca2+ accumulation in mitochondria.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00210	Rab7a; Rab7	29448	Rab7a	P09527	Rat	ER-Endosome; Endosome-ER	Endosome	PC12 cells	Low throughput experimental methods	1	25855459	Such fusion induces outgrowth of protrusions and neurites, which requires the abilities of protrudin and FYCO1 to interact with LEs and kinesin 1.Thus, protrudin-containing ER-LE contact sites are platforms for kinesin-1 loading onto LEs, and kinesin-1-mediated translocation of LEs to the plasma membrane, fuelled by repeated ER contacts, promotes protrusion and neurite outgrowth.	The protein was validated by microscopy, siRNA experiments, GFP-trap assay in PC12 cells, and these results correlate strongly with the cooperative effects of protrudin and FYCO1 in protrusion and neurite outgrowth.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00211	Zfyve27; Protrudin	309376	Zfyve27	Q6P7B7	Rat	ER-Endosome; Endosome-ER	ER	PC12 cells	Low throughput experimental methods	1	25855459	Such fusion induces outgrowth of protrusions and neurites, which requires the abilities of protrudin and FYCO1 to interact with LEs and kinesin 1.Thus, protrudin-containing ER-LE contact sites are platforms for kinesin-1 loading onto LEs, and kinesin-1-mediated translocation of LEs to the plasma membrane, fuelled by repeated ER contacts, promotes protrusion and neurite outgrowth.	The protein was validated by microscopy, siRNA experiments, GFP-trap assay in PC12 cells, and these results correlate strongly with the cooperative effects of protrudin and FYCO1 in protrusion and neurite outgrowth.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00235	Dgat2	252900	Dgat2	Q5FVP8	Rat	ER-MT; MT-ER	ER	Rat livers; McArdle-RH7777 rat hepatoma cells	Low throughput experimental methods	2	7961664	Specific activities of diacylglycerol acyltransferase, acyl-coen- zyme Acholesterol acyltransferase, and phosphatidyl- serine synthase (base exchange  enzyme) are enriched 2.2-3.4-fold in the MAM compared  with  endoplasmic re- ticulum. 	The protein was validated by immunocytochemistry, electron microscopy and conventional electron microscopy in rat livers.	19049983	The endoplasmic reticulum enzyme DGAT2 Is found in mitochondrion-associated membranes and has a mitochondrionl targeting signal that promotes its association with mitochondrion.	The protein was validated by immunoblot analyses and immunofluorescence microscopy in McArdle-RH7777 rat hepatoma cells, which N terminus may promote its association with mitochondria.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00339	Plin5; Lsdp5; Oxpat	501283	Plin5	M0R7Z9	Rat	MT-LD; LD-MT	LD; MT	Cardiac myocytes	Low throughput experimental methods	1	21885430	Perilipin 5, a lipid droplet-associated protein, provides physical and metabolic linkage to Mitochondrion.	The protein was validated by EM, micrograph analysis, immunoblot analysis, beta-oxidation measurements and statistical analysis in cardiac myocytes, which provides physical and metabolic linkage to mitochondria.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00384	Vapa; Vap33	58857	Vapa	Q9Z270	Rat	ER-Transport vesicle; Transport vesicle-ER	ER	L6 cell; 3T3-L1 cells	Low throughput experimental methods	1	11208137	A functional role for VAP-33 in insulin-stimulated GLUT4 traffic.	The protein was validated by western blotting, immunocytochemistry and microinjection/lawn assay in L6 cell and 3T3-L1 cells, and this model may be instructive for understanding the pathogenesis of HSP diseases, which may be a regulator of VAMP-2 availability for GLUT4 traffic and other vesicle fusion events.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00395	Sacm1l; Sac1	116482	Sacm1l	Q9ES21	Rat	ER-PM; PM-ER	ER	SCG neurons	Low throughput experimental methods	1	27044890	Thus, the dynamic presence of Sac1 at ER-PM contact sites allows it to act as a cellular sensor and controller of PM phosphoinositides, thereby influencing many PM processes.	The protein was validated by fluorescence microscopy, UHPLC/MS, patch-clamp recordings and photometric FRET measurements in SCG neurons, and the dynamic presence of Sac1 at ER–PM contact sites allows it to act as a cellular sensor and controller of PM phosphoinositides, thereby influencing many PM processes.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00416	Slc11a2; Dct1; Dmt1; Nramp2	25715	Slc11a2	O54902	Rat	MT-LY; LY-MT	MT	Rat kidney PT cells	Low throughput experimental methods	1	24448823	We suggest that DMT1 not only exports iron from endosomes, but also serves to import the metal into the mitochondria.	The protein was validated by split-ubiquitin yeast 2-hybrid analysis, coimmunoprecipitation, immunofluorescence microscopy and immunogold electron microscopy in rat kidney PT cells. 	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00417	Kif5b; Khc	117550	Kif5b	Q2PQA9	Rat	ER-Endosome; Endosome-ER	Endosome	PC12 cells	Low throughput experimental methods	1	25855459	Here we show in human and rat cell lines that protrudin, an ER protein that promotes protrusion and neurite outgrowth, forms contact sites with late endosomes (LEs) via coincident detection of the small GTPase RAB7 and phosphatidylinositol 3-phosphate (PtdIns(3)P). These contact sites mediate transfer of the microtubule motor kinesin 1 from protrudin to the motor adaptor FYCO1 on LEs.	The protein was validated by microscopy, siRNA experiments, GFP-trap assay in PC12 cells, and these results correlate strongly with the cooperative effects of protrudin and FYCO1 in protrusion and neurite outgrowth.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00427	Mfn2; Fzo1a	64476	Mfn2	Q8R500	Rat	ER-MT; MT-ER	MT	Rat hippocampal cells and tissues	Low throughput experimental methods	1	35090998	Lanthanum decreased VAPB-PTPP51, BAP31-FIS1, and MFN2-MFN1 expression of mitochondria-associated membranes and induced abnormal autophagy in rat hippocampus.	The protein was validated by morris water maze, transmission electron microscopy, nissl staining, immunofluorescence test , real-time quantitative PCR and western blot analysis in rat hippocampal cells and tissues.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00428	Mfn1; Fzo1b	192647	Mfn1	Q8R4Z9	Rat	ER-MT; MT-ER	MT	Rat hippocampal cells and tissues	Low throughput experimental methods	1	35090998	Lanthanum decreased VAPB-PTPP51, BAP31-FIS1, and MFN2-MFN1 expression of mitochondria-associated membranes and induced abnormal autophagy in rat hippocampus.	The protein was validated by morris water maze, transmission electron microscopy, nissl staining, immunofluorescence test , real-time quantitative PCR and western blot analysis in rat hippocampal cells and tissues.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00499	Kcnb1	25736	Kcnb1	P15387	Rat	ER-PM; PM-ER	PM	Rat hippocampal neurons	Low throughput experimental methods	2	30012696	Multiplex immunolabeling revealed colocalization of Kv2.1 and Kv2.2 with endogenous VAPs at ER-PM junctions in brain neurons from male and female mice in situ and in cultured rat hippocampal neurons, and KO of VAPA in mammalian cells reduces Kv2.1 clustering.	The protein was validated by multiplex immunolabeling in rat hippocampal neurons, live-cell tirf imaging and the Kv2.1 expression in the PM can affect ER-PM junctions via its phosphorylation-dependent association to ER-localized VAPA and VAPB.	29941597	Kv2 potassium channels form endoplasmic reticulum/plasma membrane junctions via interaction with VAPA and VAPB.	The protein was validated by immunostaining in rat hippocampal neurons, live-cell tirf imaging and the Kv2-induced ER remodeling and VAP concentration at ER/PM contacts likely play a central role in neuronal physiology.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00500	Kcnb2	117105	Kcnb2	Q63099	Rat	ER-PM; PM-ER	PM	Rat hippocampal neurons	Low throughput experimental methods	2	30012696	Multiplex immunolabeling revealed colocalization of Kv2.1 and Kv2.2 with endogenous VAPs at ER-PM junctions in brain neurons from male and female mice in situ and in cultured rat hippocampal neurons, and KO of VAPA in mammalian cells reduces Kv2.1 clustering.	The protein was validated by multiplex immunolabeling in rat hippocampal neurons, live-cell tirf imaging and the Kv2.1 expression in the PM can affect ER-PM junctions via its phosphorylation-dependent association to ER-localized VAPA and VAPB.	29941597	Kv2 potassium channels form endoplasmic reticulum/plasma membrane junctions via interaction with VAPA and VAPB.	The protein was validated by immunostaining in rat hippocampal neurons, live-cell tirf imaging and the Kv2-induced ER remodeling and VAP concentration at ER/PM contacts likely play a central role in neuronal physiology.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00505	Vapa; Vap33	58857	Vapa	Q9Z270	Rat	ER-PM; PM-ER	ER	Rat hippocampal neurons; Primary hippocampal neuron; HEK293T cells	Low throughput experimental methods	3	30012696	Multiplex immunolabeling revealed colocalization of Kv2.1 and Kv2.2 with endogenous VAPs at ER-PM junctions in brain neurons from male and female mice in situ and in cultured rat hippocampal neurons, and KO of VAPA in mammalian cells reduces Kv2.1 clustering.	The protein was validated by multiplex immunolabeling in rat hippocampal neurons, live-cell tirf imaging and the Kv2.1 expression in the PM can affect ER-PM junctions via its phosphorylation-dependent association to ER-localized VAPA and VAPB.	29941597	Kv2 potassium channels form endoplasmic reticulum/plasma membrane junctions via interaction with VAPA and VAPB.	The protein was validated by immunostaining in rat hippocampal neurons, live-cell tirf imaging and the Kv2-induced ER remodeling and VAP concentration at ER/PM contacts likely play a central role in neuronal physiology.	36769244	Importantly, we demonstrate that proNRG2 promotes the formation of ER-PM junctions by directly binding the ER-resident membrane tether VAP, like Kv2.1.	The protein was validated by shRNA-mediated knockdown, immunocytochemistry, confocal microscopy, immunogold electron microscopy, immunoprecipitation, GST pulldown assays and western blotting in primary hippocampal neuron. ProNRG2 and Kv2.1 serve as co-regulated downstream effectors of NMDA receptors to homeostatically regulate GABAergic interneurons.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00506	Vapb	60431	Vapb	Q9Z269	Rat	ER-PM; PM-ER	ER	Rat hippocampal neurons; Primary hippocampal neuron; HEK293T cells	Low throughput experimental methods	3	30012696	Multiplex immunolabeling revealed colocalization of Kv2.1 and Kv2.2 with endogenous VAPs at ER-PM junctions in brain neurons from male and female mice in situ and in cultured rat hippocampal neurons, and KO of VAPA in mammalian cells reduces Kv2.1 clustering.	The protein was validated by multiplex immunolabeling in rat hippocampal neurons, live-cell tirf imaging and the Kv2.1 expression in the PM can affect ER-PM junctions via its phosphorylation-dependent association to ER-localized VAPA and VAPB.	29941597	Kv2 potassium channels form endoplasmic reticulum/plasma membrane junctions via interaction with VAPA and VAPB.	The protein was validated by immunostaining in rat hippocampal neurons, live-cell tirf imaging and the Kv2-induced ER remodeling and VAP concentration at ER/PM contacts likely play a central role in neuronal physiology.	36769244	Importantly, we demonstrate that proNRG2 promotes the formation of ER-PM junctions by directly binding the ER-resident membrane tether VAP, like Kv2.1.	The protein was validated by shRNA-mediated knockdown, immunocytochemistry, confocal microscopy, immunogold electron microscopy, immunoprecipitation, GST pulldown assays and western blotting in primary hippocampal neuron. ProNRG2 and Kv2.1 serve as co-regulated downstream effectors of NMDA receptors to homeostatically regulate GABAergic interneurons.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00510	Sigmar1; Oprs1	29336	Sigmar1	Q9R0C9	Rat	ER-MT; MT-ER	ER	Microglial cells 	Low throughput experimental methods	1	34601668	Sigma-1R (σ-1R) localizes to the mitochondria-associated membranes (MAMs) and regulates endoplasmic reticulum (ER) and mitochondria communication, in part through its chaperone activity.	The protein was validated by TEM, flow cytometry analysis, immunofuorescent staining, confocal microscopic imaging, western blot and Real‑Time RT‑PCR in microglial cells, which suppresses microglia M1 polarization and neuroinfammation via regulating endoplasmic reticulum–mitochondria contact and mitochondrial functions in stress-induced hypertension rats.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00525	Lonp1; Lon; Prss15	170916	Lonp1	Q924S5	Rat	ER-MT; MT-ER	MT	H9C2 cells	Low throughput experimental methods	1	37333972	In the present study, we first identify LonP2 as a new MAM-localized protein that links ER and mitochondria in the cardiomyocytes.	The protein was validated by staining, transmission electron microscopy, western blot analysis and fluorescence detection in H9C2 cells, and LonP1 deficiency in the heart results in aberrant metabolic reprogramming and pathological heart remodeling and eventually progresses to heart failure.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00557	Fundc1	363442	Fundc1	Q5BJS4	Rat	ER-MT; MT-ER	MT	H9C2 cells	Low throughput experimental methods	1	35118141	More importantly, we found STAT3-knockdown suppressed LPS-induced expression of FUNDC1, a protein located in mitochondria-associated endoplasmic reticulum membranes (MAMs).	The protein was validated by cell viability assay, immunofluorescence staining, transfection, assessment of ROS in H9C2 cells, and IL-6/STAT3 pathway plays a key role in LPS-induced myocardial dysfunction, through regulating the FUNDC1-associated MAMs formation and interfering the function of ER and mitochondria.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00578	Nrg2; Ntak	432361	Nrg2	O35569	Rat	ER-PM; PM-ER	PM	Primary hippocampal neuron	Low throughput experimental methods	1	36769244	Importantly, we demonstrate that proNRG2 promotes the formation of ER-PM junctions by directly binding the ER-resident membrane tether VAP, like Kv2.1.	The protein was validated by shRNA-mediated knockdown, immunocytochemistry, confocal microscopy, immunogold electron microscopy, immunoprecipitation, GST pulldown assays and western blotting in primary hippocampal neuron. ProNRG2 and Kv2.1 serve as co-regulated downstream effectors of NMDA receptors to homeostatically regulate GABAergic interneurons.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00588	Pdzd8	308000	Pdzd8	D3ZXY2	Rat	ER-Endosome; Endosome-ER	NA	PC12 cells	Low throughput experimental methods	1	33912962	PDZD8-mediated lipid transfer at contacts between the ER and late endosomes/lysosomes is required for neurite outgrowth.	The protein was validated by confocal microscopy, live cell super-resolution Lattice-SIM microscopy, immunofluorescence staining, quantitative RT-PCR, GFP-trap assay, mass spectrometry, pulldown assays and pull-down assay in PC12 cells, which is required for LE/lys positioning and neurite outgrowth, which is dependent on the lipid transfer activity of the SMP domain.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00589	Pdzd8	308000	Pdzd8	D3ZXY2	Rat	ER-LY; LY-ER	NA	PC12 cells	Low throughput experimental methods	1	33912962	PDZD8-mediated lipid transfer at contacts between the ER and late endosomes/lysosomes is required for neurite outgrowth.	The protein was validated by confocal microscopy, live cell super-resolution Lattice-SIM microscopy, immunofluorescence staining, quantitative RT-PCR, GFP-trap assay, mass spectrometry, pulldown assays and pull-down assay in PC12 cells, which is required for LE/lys positioning and neurite outgrowth, which is dependent on the lipid transfer activity of the SMP domain.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA
LMCS00632	Fyco1	301085	Fyco1	D3Z9D2	Rat	ER-Endosome; Endosome-ER	Endosome	PC12 cells	Low throughput experimental methods	1	25855459	Here we show in human and rat cell lines that protrudin, an ER protein that promotes protrusion and neurite outgrowth, forms contact sites with late endosomes (LEs) via coincident detection of the small GTPase RAB7 and phosphatidylinositol 3-phosphate (PtdIns(3)P). These contact sites mediate transfer of the microtubule motor kinesin 1 from protrudin to the motor adaptor FYCO1 on LEs.	The protein was validated by microscopy, siRNA experiments, GFP-trap assay in PC12 cells, and these results correlate strongly with the cooperative effects of protrudin and FYCO1 in protrusion and neurite outgrowth.	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA	NA